M.A. Starostina1, Z.A. Afanaseva2, R.A. Starostin3
1Tatarstan Cancer Center, Kazan
2Kazan State Medical Academy ― Branch Campus of the FSBEI FPE RMACPE MOH Russia, Kazan
3Kazan State Medical University, Kazan
Afanaseva Zinaida A. ― Doct. of Sci. (Med.), Professor of the Department of oncology, radiology and palliative medicine of the Kazan State Medical Academy ― Branch Campus of the FSBEI FPE RMACPE MOH Russia
36 Butlerov Str., Kazan, 420012, Russian Federation, tel. +7-917-886-29-23, e-mail: firstname.lastname@example.org
Aim ― in this analysis, we compare the results of our own research on the use of non-invasive tests MMP-9, TIMP-1, fTu M2-PK, Hexagon OBTI hemocultest, determined in coprofiltrates, and their combinations in the diagnosis and screening of colorectal cancer.
Material and methods. As a material, we used 63 colorectal cancer patients’ stool samples taken prior to treatment. The control group represented by 20 healthy comparison subjects. 1071 persons underwent colorectal cancer screening.
MMP-9, TIMP-1, fTu M2-PK, Hexagon OBTI were used as diagnostic tests, Hexagon OBTI and TIMP-1 – as screening tests. ELISA using Quantikine ELISA kits (R&D Systems, USA) estimated MMP-9 and TIMP-1 levels in coprofiltrates, fTu M2-PK were estimated using enzyme immunoassay test systems: ScheBo Tu M2-PKTM ELISA Stool Test (ScheBo Biotech AG, Germany), quick fecal occult blood analysis ― Hexagon OBTI (HUMAN, Germany). Sensitivity, specificity, accuracy of these tests and their combinations were estimated.
Results. MMP-9 and TIMP-1 levels in coprofiltrates of colorectal cancer patients were significantly higher than those of the control group were and did not depend on the stage of the disease and the tumor localization. TIMP-1 and Hexagon OBTI turned out as informative tests for colorectal cancer diagnosis with sensitivity, specificity and accuracy of 76,2%, 95%, 80,7% and 86,2%, 93,8%, 87,7%, respectively. The level of fTu M2-PK in coprofiltrates of patients with colorectal cancer did not differ from the level of fTu M2-PK of the control group, which prevents the use of it as a non-invasive diagnostic test. The use of a combination of TIMP-1 and Hexagon OBTI tests for colorectal cancer screening increased their informativity: sensitivity increased to 100%, specificity to 97,3%, accuracy to 97,3%.
Conclusion. The informativity of application of Hexagon OBTI faecal occult blood test increases when applicated in combination with TIMP-1, thus it is relevant to use their combination as non-invasive tests for colorectal cancer screening.
Key words: colorectal cancer, molecular markers, Hexagon OBTI, diagnosis, screening.