Ph.S. Bova1, O.I. Kit2, A.Yu. Maksimov2, N.S. Karnaukhov2
1The Regional Hospital №2, Rostov-on-Don
2Rostov Research Oncological Institute, Rostov-on-Don
Bova Ph.S. ― Head of the Center for Urology, Nephrology and Hemodialysis of the Regional Hospital №2
33 the 1st Cavalry Army Str., Rostov-on-Don, Russian Federation, 344112, tel. (863) 254-98-90, +7-928-957-08-06, e-mail: alald@inbox.ru
Abstract. The measurement of gene copy is important for determining the molecular mechanisms of the progression of cancer. The aim of the study was to study the relative replicability of genetic loci responsible for apoptosis and control of neoangiogenesis in tumor tissues of the prostate gland in patients with localized prostate cancer (PC) with biochemical recurrence (BR) and without recurrences after radical prostatectomy. The main group included 56 patients with localized PC who had been diagnosed with BR within two years after RP. 60 patients with localized PC who did not relapse had a comparative group. 55 patients in whom operative biopsy specimens of the prostate gland were taken within healthy tissues with the removal of benign prostatic hyperplasia were combined into a control group. Determination of the relative copies of the genetic loci BAX, BCL2, VEGFA, VEGFB was performed by real-time PCR. It was found that a significant increase in the activity of the gene stimulating neoangiogenesis was observed in the patients of the main group in tumor samples, as well as inhibition of apoptosis due to an increase in the expression of the BCL2 gene. The decrease in the ratio characterizing the ratio of the relative copies of genes with the pro-apoptotic and antiapoptotic activity of BAX/BCL2 below 1,64 is associated with a risk of developing BR in patients with localized PC with a diagnostic sensitivity of 89,3% and a specificity of 88,3%.
Key words: prostate cancer, biochemical relapse, prognosis, gene copy, apoptosis, neoangiogenesis.